Cyanine 3-COOH on FRET (fluorescence resonance energy transfer) Application of the cyanine dye CY3-carboxylate Protein molecular conformation

CY3-COOH has important applications in FRET (Fluorescence Resonance Energy Transfer) technology, a phenomenon based on non-radiative energy transfer between fluorescent molecules, where signal transmission and amplification can be achieved by combining two fluorescent dyes, Donor and Acceptor.CY3-COOH is commonly used as a Donor dye and plays an energy transfer role in FRET. FRET to play the role of energy transfer. The following are the applications of CY3-COOH in FRET and the corresponding examples:

1. Protein interaction studies:

The study of the interaction of these two proteins can be achieved by labeling CY3-COOH on one protein and then another protein that interacts with it on the Acceptor dye. When the two proteins bind, the fluorescence energy of CY3-COOH will be transferred to the Acceptor dye through FRET, producing the fluorescence signal of the Acceptor. This method can be used to study protein interactions, binding kinetics and spatial structure.

2. Intermolecular distance measurements:

By labeling CY3-COOH and Acceptor dye on two molecules, the distance between them can be measured. When the distance between the two molecules is less than the FRET effective range, the fluorescence energy of CY3-COOH will be transferred to the Acceptor dye via FRET, generating the fluorescence signal of Acceptor. Based on the properties of the FRET effect, the distance between the two molecules can be inferred from the intensity of the fluorescent signal of Acceptor and the intensity of the fluorescent signal of CY3-COOH.

3. Molecular conformational studies:

By labeling CY3-COOH on a molecule, it is possible to study the conformational changes of the molecule. When a molecule undergoes a conformational change, the fluorescence properties of CY3-COOH also change, thereby affecting the intensity and efficiency of the FRET effect. By monitoring the fluorescence signal of Acceptor and the fluorescence signal of CY3-COOH, it is possible to speculate on the conformational change of the molecule.

In conclusion, CY3-COOH has important applications as Donor dye in FRET technology. By combining with Acceptor dyes, the study of protein interactions, intermolecular distances and molecular conformations can be realized. These applications provide powerful tools for studying intermolecular interactions, molecular structure and function, etc.